Separation and extraction of lutein
1, membrane separation technology.
Ceramic membrane microfiltration (MF) to extract the fine filter purification, and then the reverse osmosis membrane (R0) of concentrated filtrate. This process of membrane separation technology as the main alternative to the traditional alcohol purification and evaporation, the process is simple, the pigment solution in the basic operating state at room temperature, which saves energy, but also to ensure the quality of the product of pigment.
2, the drying method to extract lutein.
Developed a new tumble dryer used to dry and thump calendula or marigold petals, can be extracted from lutein. When the thump ratio, thump efficiency fluctuated between 70% to 90%. Lutein how much depends on the length of the drying time. However, in the same drying time, the lutein content of the dried extract under 70 ℃ less than 60 ℃ extract lutein content.
3, HPLC analysis was extracted.
Dichloromethane (22.5mL) + acetonitrile (9.5mL) + methanol (67.5mL) + water (0.5mL of) as the mobile phase, flow rate was 1.0mL/min. Photometric detector (wavelength 450nm) detection after the separation of carotenoids and chlorophyll. The sample injection volume of 20μL. Under these conditions, lutein, chlorophyll b, chlorophyll a, lycopene (lycopene), α-carotene and beta-carotene retention time were 3.0,4.9,6.6,12.1,14.6 and 15.3min. Take spirulina culture medium (obtained by measuring the optical density (560nm) to cultivate Spirulina dry weight of the liquid in the centrifuge tube after centrifugation, discard the solution, add water washing after adding 2mL volume fraction of 90% aqueous acetone, placed in ultrasonic generator on a water bath, so that Spirulina broken. After centrifugation, the upper extract by HPLC determination of lutein / zeaxanthin, beta-carotene and chlorophyll a content, the highest contents were: 1.65μg / mg 2.15μg/mg and the 21.4μg/mg.